Figure 4

EI-03 regulates T cell differentiation. Naïve CD4⁺ T cells were differentiated into Th17 with IL-6 (20 ng/ml) and TGFβ (1 ng/ml) or Th1 with IL-12 (10 ng/ml) for 3 days in the presence or absence of EI-03 (10 μM). Production of IL-17 (A) and IFNγ (B) was measured by intracellular flow staining. Lymphocytes from LNs were cultured with anti-CD3/CD28 antibody in the presence or absence of EI-03 (10 μM) for 3 days. Foxp3 (C) and IFNγ (D) in CD4⁺ T cells were examined by intracellular staining. (E) Naïve CD8⁺ T cells were differentiated with IL-12 (20 ng/ml) in the presence or absence of EI-03 (10 μM) for 3 days. IFNγ production was examined by intracellular flow staining. Each panel displays a representative of three experiments yielding similar results. Data are presented as the means ± SD.