Figure 5
Amelioration of EAE is associated with reduced leucocyte infiltration and effector T cell function in EI-03-treated mice. (A) C57B/6 mice treated with EI-03 or DMSO vehicle control (n = 9/group) were immunized with MOG35–55 and scored daily postimmunization (*, p < 0.05). (B) EAE mice from EI-03-treated or control mice were sacrificed at the peak of the disease and total numbers of mononuclear cells in the CNS of those mice were measured with an automatic cell-counter. Percentage of infiltrated CD4+ T cells (C), CD8+ T cells (D) and CD11b+MHCII+ cells (E) were analyzed by flow cytometric staining. The expression of IL-17, IFNγ (F) and Foxp3 (G) in CNS-infiltrated CD4+ T cells were determined using intracellular staining. (H) Total numbers of IFNγ+ CD8+ T cells in CNS were measured using intracellular staining. The results shown are representative of three independent experiments (*, p < 0.05).