Figure 3
Inhibition of proliferation of T lymphocyte subpopulations in right inguinal nodes in EAE mice following OGF or LDN treatment. Total lymphocytes isolated from right inguinal nodes of mice immunized with MOG35–55 and injected daily with 10 mg/kg OGF (OGF), 0.1 mg/kg naltrexone (LDN), or 0.1 ml saline (saline); normal mice received saline at each injection point. Right nodes were activated following injections on day 0; lymphocytes from 3–4 mice per treatment at 5 and 12 days were isolated, labeled, and sorted by flow cytometry. Values represent means ± SEM. Significantly different from normal mice at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***), and from saline-treated immunized mice at p < 0.05 (+).