Fig. 1
From: Inhibition of RORγt activity and Th17 differentiation by a set of novel compounds
Validation of the RORγt-LBD+ Jurkat stable cell line. RORγt-LBD+ Jurkat cells were seeded into 96-well round-bottom plates (2 × 104) and cultured overnight prior to incubation with the positive control compound (CAS registry number is 744267-30-9; 1a) at 5-fold gradient dilutions with final concentrations ranging from 5 μM to 8 nM for 6 h. Cells were lysed 6 h later and assayed for luciferase activity. The EC50 value of the 744267-30-9 was 723nM based on the relative luciferase activity (1b). The results were shown as the means ± SEM