Fig. 3

γδ T lymphocytes migrate from spleen into the lungs of CLP-operated recipient mice. T lymphocytes recovered from the spleen of naïve mice were labeled with CFSE and transferred to CLP-operated mice 3 and 8 days after surgery. Recipient animals were euthanized 10 days after surgery, and their lungs, blood and spleen were collected for γδ (a–c) and αβ (d–f) T cell analysis by flow cytometry. Quantification of CCL2, CCL3, and CCL5 levels in lung homogenates of naïve, sham and CLP C57BL/6 mice by ELISA, 7 days after surgery (g). γδ T cell chemotaxis towards lung homogenates from CLP mice (or towards CCL2, CCL3 and CCL5), incubated or not with neutralizing α-CCL2, α-CCL3 or α-CCL5, as described in methods (h). Representative results of two experiments from at least 4 animals per experimental group are expressed as mean ± SEM. Statistical differences (p < 0.05) between CLP and sham groups are indicated by (*), and between stimulated and mAb-treated groups are indicated by (+)