Fig. 4

Increased IL-17 production by Vγ4 T lymphocytes in CLP mouse lungs. a Percentages of γδ and αβ T lymphocytes among lung IL-17⁺ T cells recovered 10 days after CLP, as determined by intracellular staining. b Representative dot plots of intracellular staining of IL-17⁺ within γδ, Vγ4 and Vγ1 T cells recovered from the lungs of CLP mice. c Percentages of CD27^- cells among γδ, Vγ4 and Vγ1 T cell population in the spleen recovered 3 days after CLP. d Representative histograms of CD27 staining of γδ, Vγ4 and Vγ1 T cells recovered from mouse spleen. e IL-17 quantification in lung homogenates of CLP mice treated or not with α-γδ TCR mAb, α-Vγ4 TCR mAb or control IgG, 7 days after surgery was performed by CBA. Results are expressed as mean ± SEM from at least 5 animals per group. Statistical differences (p < 0.05) between CLP and sham groups are indicated by (*), and between stimulated and mAb-treated groups are indicated by (+)