The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells

Table 1 Statistical assessment of the amplification performance of 11 candidate genes in bovine blood mononuclear cells

Gene*	Slope	StdErr _s	P value _s	Intercept	StdErr _i	P value _i	R ²	E
18s	−5.06151	0.0758	7.586E-10	25.3305	0.14632	2.507E-12	0.99866	0.57605
24s	−3.79032	0.05825	8.863E-10	31.739	0.11246	1.335E-13	0.99858	0.83582
ACTB	−3.368	0.064719	3.379E-09	19.8495	0.12108	3.475E-12	0.99779	0.98113
B2M	−3.93206	0.01988	1.127E-12	31.9222	0.03838	2.038E-16	0.99985	0.79606
CTBP1	−3.28926	0.09434	3.7E-08	35.1283	0.18211	1.31E-12	0.99509	0.98619
GAPDH	−3.46422	0.03558	7.917E-11	31.7187	0.0687	6.966E-15	0.99937	0.94386
MDM4	−3.73883	0.0703	3E-09	38.6855	0.13572	1.258E-13	0.99788	0.85124
PPIA	−4.31421	0.027696	4.722E-12	35.6981	0.058222	1.27E-15	0.99975	0.70527
RAD50	−3.94515	0.1124	4E-09	39.6633	0.20941	3.018E-14	0.99435	0.79257
SDHA	−3.69503	0.1683	1.03E-07	36.2313	0.30674	8.221E-13	0.98569	0.86481
YWHAZ	−3.55114	0.03416	5.343E-11	32.1879	0.06595	4.993E-15	0.99945	0.91249

Slope: Slope of standard curve; StdErr_s: Standard error of the slope; P_value_s: Statistic significance of the slope estimation; Intercept: Intercept of standard curve; StdErr_i: Standard error of the intercept; P value_i: Statistic significance of the intercept estimation; R²: the correlation coefficient; E: Efficiency of amplification. * 18s: 18S ribosomal RNA; 24s: Ribosomal protein S24; ACTB: Actin, beta; B2M: Beta-2-microglobulin; CTBP1: C-terminal binding protein 1; GAPDH: Glyceraldehydes-3-phosphate dehydrogenase; MDM4: mouse double minute 4 protein; PPIA: Peptidylprolyl isomerase A; RAD50: RAD50 homolog (S. cerevisiae); SDHA: Succinate dehydrogenase complex, subunit A; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monoxygenase activation protein, zeta polypeptide.

ISSN: 1471-2172