The effect of immunological status, in-vitro treatment and culture time on expression of eleven candidate reference genes in bovine blood mononuclear cells

Table 2 Probabilities of significance of fixed effects on the expression of candidate housekeeping genes in bovine blood mononuclear cells under different in-vitro treatment. Significant probabilities (P ≤ 0.05) are shown in bold

Candidate gene symbol*		24s	CTBP1	MDM4	PPIA	RAD50
Source of variation
Mitogenic treatment (ConA)
	Immunological Status^#	0.2936	0.8759	0.5817	0.1220	0.9072
	Culture time^§	0.2345	0.0041	0.0213	0.0002	<.0001
	Immunological Status*Culture time	0.4287	0.4041	0.0036	0.5697	0.019
Antigenic treatment
Complex antigen (C. albicans)
	Immunological Status^#	0.3501	0.6967	0.0032	0.1602	0.0524
	Culture time^§	0.0420	0.0179	0.3493	0.0007	0.0009
	Immunological Status *Culture time	0.4111	0.6621	0.0161	0.0148	0.8384
Single polypeptide (HEWL)
	Immunological Status^#	0.0578	0.3333	0.0417	0.1948	0.0415
	Culture time^§	0.0970	0.1510	0.9049	0.0055	0.6798
	Immunological Status *Culture time	0.2852	0.2769	0.2026	0.8707	0.6076

*24s: Ribosomal protein S24; CTBP1: C-terminal binding protein 1; MDM4: mouse double minute 4 protein; PPIA: Peptidylprolyl isomerase A; RAD50: RAD50 homolog (S. cerevisiae). # General effect of immunological status includes naïve, immunized, and boosted groups. § General effect of culturing time included 4 and 24 hour time points. Bonferroni correction was not applied to this analysis since multiple comparison was not appropriate for all the fixed effect in this model

ISSN: 1471-2172