Table 3 Candidate genes characteristics and primers sequence

18s

18S ribosomal RNA

Ribosome unit

F: 5'-AGA AAC GGC TAC CAC ATC -3'

Primer3web

R: 5'-GGA CTC ATT CCA ATT ACA GG -3'

24s

Ribosomal protein S24

Ribosome unit

F: 5'-TGT CAT CTT TGT ATT TGG GTT CAG -3'

Primer3web

R: 5'-TCT GTT CTT GCG TTC CTT CC -3'

ACTB

Actin, beta

Structural protein

F: 5'- GCT TCT AGG CGG ACT GTT AG -3'

Primer3web

R: 5'-ACT TGG GAA TGC TCG ATC C -3'

Β2M

Beta-2-microglobulin

Beta chain of MHC I

F: 5'-TTA CCT GAA CTG CTA TGT GTA TG -3'

Primer3web

R: 5'-CTG TAC TGA TCC TTG CTG TTG -3'

CTBP1

C-terminal binding protein 1

Involved in cellular proliferation

F: 5'-ACA ACC ACCACC TCA TCA AC -3'

Primer3web

R: 5'-AGC CTT CTC GTC CAC CAG -3'

GAPDH

Glyceraldehydes-3-phosphate dehydrogenase

Glycolysis and gluconeogenesis

F: 5'-GTT CAA CGG CAC AGT CAA G -3'

Primer3web

R: 5'-TAC TCA GCA CCA GCA TCA C -3'

MDM4

mouse double minute 4 protein

p53 binding protein, Housekeeping gene in Th1 cells

F: 5'-GGA GAA CTA CTA GGT CGT CAG AG -3'

Primer3web

R: 5'-CCT GTG CGA TAG CGA GAG TC -3'

PPIA

Peptidylprolyl isomerase A

Protein folding

F: 5'-TGA CTT CAC ACG CCA TAA TGG T -3'

Bevilacqua C. et al. 2006 [30]

R: 5'-CAT CAT CAA ATT TCT CGC CAT AGA -3'

RAD50

RAD50 homolog (S. cerevisiae)

DNA double-strand break repair, Housekeeping gene in Th2 cells

F: 5'-CAC AAT CAC CAG AGA ACA GTA AGG -3'

Primer3web

R: 5'-CTG ATG ACG ATC TGC TTG TAG TTG -3'

SDHA

Succinate dehydrogenase complex, subunit A

Electron transporter

F: 5'-CGT TGT ATG GAA GGT CTC TG -3'

Primer3web

R: 5'-GTG GCG ATG ACA GTG TTC -3'

YWHAZ

Tyrosine 3-monooxygenase/tryptophan 5-monoxygenase activation protein, zeta polypeptide

Signal transduction by binding to phosphoserine-containing proteins

F: 5'-GAC TAC TAC CGCTAC TTG GCT GAG -3'

Primer3web

R: 5'-GCT TCT TGG TAT GCT TGC TGT GAC -3'