A study on immunomodulatory mechanism of Polysaccharopeptide mediated by TLR4 signaling pathway

BMC Immunology

Table 1 Sequence and useful information of primers designed for detection of mRNA

Gene symbol	Genbank accession no.	Primer sequence (5^’ → 3^’)	Product size (bp)	Annealing temperature	E (%)	R²
TLR4	NM_021297.2	F ctgggtgagaaatgagctggtaa	122	58 °C	96.5	0.999
TLR4	NM_021297.2	R agccttcctggatgatgttgg	122	58 °C	96.5	0.999
TRAF6	NM_009424	F catcttcagttaccgacagctcag	131	58 °C	95.2	0.999
TRAF6	NM_009424	R tggtcgagaattgtaaggcgtat	131	58 °C	95.2	0.999
NF-κB	NM_008689.2	F ccaaagaaggacacgacagaatc	127	58 °C	95.5	0.999
NF-κB	NM_008689.2	R ggcaggctattgctcatcaca	127	58 °C	95.5	0.999
AP-1	NM_010591.2	F gccctggctgaactgcatag	180	58 °C	91.4	0.998
AP-1	NM_010591.2	R gaagttgctgaggttggcgta	180	58 °C	91.4	0.998
GAPDH	NM_008084.2	F gacatcaagaaggtggtgaagc	117	58 °C	96.2	0.999
GAPDH	NM_008084.2	R gaaggtggaagagtgggagtt	117	58 °C	96.2	0.999
β-Actin	NM_007393.3	F agattactgctctggctcctagc	147	58 °C	93.6	0.998
β-Actin	NM_007393.3	R actcatcgtactcctgcttgct	147	58 °C	93.6	0.998

F Forward primer, R Reverse primer, E Amplification efficiency, R ² Correlation coefficient

ISSN: 1471-2172