Skip to main content
Fig. 2 | BMC Immunology

Fig. 2

From: Mycobacterial glycolipids di-O-acylated trehalose and tri-O-acylated trehalose downregulate inducible nitric oxide synthase and nitric oxide production in macrophages

Fig. 2

DAT and TAT downregulate NO production induced by LPS and IFN-γ in bone marrow-derived MØs. The isolated TAT or DAT were dissolved in hexane/methanol and placed in the wells. After solvent evaporation 1 × 106, MØs were added to the wells. Afterward LPS (500ng) or IFN-γ (250ng) were added to the cells. After 24 h, the culture medium was collected, and NO was measured by the Griess reaction (a, c). The percent inhibition of NO production induced by TAT (b) and DAT (d) are shown. Results of four experiments are presented. Data regarding LPS against DAT/LPS was analyzed using an unpaired t-test with Welch’s correction to assess the statistical significance *p < 0.05

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us