Fig. 2
Effects of DEX on PD-1 expression on activated T cells. a Mouse T cells were incubated with anti-CD3/CD28 beads for 48 h in the absence or presence of various doses of DEX (10−9-10−5 M). b Mouse T cells were incubated with anti-CD3/CD28 beads in the absence or presence of 1 × 10−7 M DEX for 0 to 7 days. DEX significantly increased PD-1 expression. c DEX increased PD-1 expression in both CD4+ and CD8+ subsets of mouse T cells. d DEX increased PD-1 expression in both CD4+ and CD8+ subsets of human T cells. e Analysis of PD-1 mRNA level in DEX-treated and untreated T cells by real-time PCR analysis. Data are presented as the mean fold change in PD-1 mRNA level normalized to the β-actin mRNA. f Western blot analyses confirming higher expression of PD-1 protein in DEX-treated T cells. Data represents mean ± SEM of three experiments. *p < 0.05. **p < 0.01