Fig. 4

Different T cell subsets differ in their expression of PD-1 and sensitivity to DEX. Cells were incubated with anti-CD3/CD28 beads for 48 h in the absence or presence of 1 × 10⁻⁷ M DEX. a Based on the expression of CD44 and CD62L, four subsets were gated: CD44⁻CD62L⁺ (naïve T cells, Tn), CD44⁺CD62L⁺ (central memory T cells, Tcm), CD44⁺CD62L⁻ (effector memory T cells, Tem) and CD44⁻CD62L⁻ (double negative T cells). b Representative flow cytometry histograms show PD-1expression differs in Tn, Tcm and Tem, with Tem expressing the highest level and Tn the lowest. c Tcm, Tem are more susceptible to DEX-induced upregulation of PD-1. Data is presented as mean ± SEM of three experiments. **p < 0.01