Fig. 6
Effects of DEX on the function and apoptosis of activated T cells. Activated mouse T cells were cultured with DEX (10−7 M) or medium for 48 h. a Culture supernatants were collected and assayed for IL-2, TNF-α and IFR-γ. Data are shown as the mean values (±SEM) for triplicate cultures for each experiment. The results are from two independent experiments. Statistically significant differences between medium alone and DEX-treated groups was evaluated at *p < 0.05 or **p < 0.01. b Following 48 h incubation, cells were analyzed for apoptosis by FITC-Annexin V and PI staining by flow cytometric analysis. Annexin-V-, PI- cells were live cells, Annexin-V+, PI- cells were early apoptotic cells, Annexin-V+, PI+ cells were late apoptotic and dead cells were PI positive. c The percentages of apoptotic cells were labeled as Annexin V+ cells. Data is presented as mean ± SEM of three experiments. **p < 0.01