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Table 2 Gliadin content quantified in the apical and basal chamber of Caco-2 cell cultures exposed to digested gliadin with/without anti-wheat gliadin

From: Effect of anti-gliadin IgY antibody on epithelial intestinal integrity and inflammatory response induced by gliadin

Condition

% Gliadin

Apical

Basal

1 h

2 h

4 h

1 h

2 h

4 h

A

ND

ND

ND

ND

ND

ND

C

98 ± 3

90.3 ± 3

76.3 ± 3*

ND

2.5 ± 0.5

14.8 ± 1**

D

ND

ND

ND

ND

ND

ND

E

96.2 ± 1

93.5 ± 1

94.2 ± 1

ND

ND

ND

F

94.0 ± 1

93.7 ± 1

94.9 ± 1

ND

ND

ND

G

97 ± 1

94.9 ± 1

94.5 ± 1

ND

ND

ND

  1. A: Phosphate buffered saline (PBS)
  2. C: Pepsin-trypsin digested gliadin (PT-gliadin)
  3. D: anti-wheat gliadin
  4. E: PT-gliadin: anti-gliadin IgY (1:6,000)
  5. F: PT-gliadin: anti-gliadin IgY (1:3,000)
  6. G: PT-gliadin: anti-gliadin IgY (1:1,500)
  7. Data from a representative experiment performed are shown as mean ± SD (n = 15)
  8. * indicate statistically significant lower gliadin content in apical chamber (P < 0.05)
  9. ** indicate statistically significant higher gliadin content in basal chamber (P < 0.05)
  10. ND: Not detectable
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BMC Immunology

ISSN: 1471-2172

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