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Fig. 3 | BMC Immunology

Fig. 3

From: IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus

Fig. 3

Cell cultures stimulated with rIFNγ only. Unseparated PBL of 4 healthy individuals were cultured for 48 h in the presence of 500 ng/ml, 1000 ng/ml or without rIFNγ. There was no significant increase of IFNγ+CD4+CD25+Foxp3+CD127- Treg during the culture period (24 h resp. 48 h: 0 ng/ml vs 500 ng/ml resp. 1000 ng/ml, p = n.s.). The data suggest that rIFNγ is unable to induce Treg, especially IFNγ+CD4+CD25+Foxp3+CD127- Treg, without an additional stimulus. Rather, there was a decrease of CXCR3+CD62L+ (48 h: 0 ng/ml vs 500 ng/ml resp. 1000 ng/ml rIFNγ, both p = 0.029) as well as CD152+CD154+ (48 h: 0 ng/ml vs 1000 ng/ml rIFNγ, p = 0.029) Treg. Data are given as mean ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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BMC Immunology

ISSN: 1471-2172

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