Fig. 5
From: In silico prediction of Ebola Zaire GP1,2 immuno-dominant epitopes for the Balb/c mouse
Tetramer staining of immunized splencocytes. Splenocytes from peptide immunized animals were stained with KD tetramers prepared with P8, P9, P10 or Adjuvant (mock-infected). The cells were counter stained with CD8 FITC and CD3 PerCP, then fixed. The stained cells were analyzed in a FACS ARIA. Tetramer positive cells were sorted and re-analyzed. Pre-sort and Post sorted tetramer positive cells are shown in figure. The results are representative of three independent experiments