Immunogenicity of twenty peptides representing epitopes of the hepatitis B core and surface antigens by IFN-γ response in chronic and resolved HBV

Table 1 HLA class I restricted epitopes (I)

Number	Residues	Sequence	HLA	Reference
HBsEC-1	20–28	FLLTRILTI	A*02:01, 02:05, 02:06	[13]
HBsEC-2	88–96	LLCLIFLLV	A2	[9, 14]
HBsEC-3	95–104	LVLLDYQGML	A2	[8]
HBsEC-4	172–180	WLSLLVPFV	A*02:01, 02:02,02:03,02:05, 02:06	[6, 8, 9, 14, 15]
HBsEC-5	207–216	SIVSPFIPLL	A2	[14]
HBsCP-6^b	169–176	FLGPLLVL	A2^a
	180–187
	179–188
HBsCP-7^b	72–79	LLGWSPQA	A2^a
	61–68
	71–78
HBsCP-8^b	259–266	LLLCLIFL	A2^a
	248–255
	258–265
HBsCP-9^b	333–370	FLWEWASA	A2^a
	332–339
	322–329
HBsCP-10^b	129–136	PAGGSSSG	A2^a
	140–147
	139–146
HBcEC-11	18–27	FLPSDFFPSV	A*02:01, 02:05, 02:06	[6, 8, 9, 14–17]
HBcEC-12	88–96	YVNVNMGLK	A11	[18]
HBcEC-13	107–115	CLTFGRETV	A2	[8]
HBcEC-14	117–125	EYLVSFGVW	A24	[19]
HBcEC-15	139–148	ILSTLPETTV	A2	[8]
HBcCP-16	141–148	TLPETTVV	A2
HBcCP-17	160–168	PSPRRRRS	A2
HBcCP-18	64–71	LMTLATWV	A2
HBcCP-19	29–36	LLDTASAL	A2
HBcCP-20	107–114	LTFGRETV	A2

Name and amino acid position of the 20 epitopes. HLA genotype indicates which genotypes were tested in previous studies (HBsEC1-5 and HBcEC11-15), or which genotypes we stratified for in the in silico analysis (HBsCP6-10 and HBcCP16-20)
HBsEC HBsAg experimentally confirmed, HBsCP HBsAg computer predicted, HBcEC HBcAg experimentally confirmed, HBcCP HBcAg computer predicted
^aall subtypes
^bdifferences of sequence position among HBV genotypes was observed for in silico prediction of HBsAg

ISSN: 1471-2172