Fig. 6
Validation of sRANKL-LAB as oral adjuvant in vivo. a Schematic view of sRANKL-LAB treatment, priming-boosting and sampling schedule (n = 5 in each group tested). b M-BmpB-specific total serum IgG levels at days of 21 and c 28, and d IgG1 subtype and e IgG2a subtype at day 28 after priming were determined by ELISA and expressed as OD value (450 nm). f M-BmpB-specific fecal IgA levels at days of 21 and g 28, and h intestinal IgA levels at day 28 were measured with the same method. PBS/PBS; oral administration with PBS for every 7 days and immunization with PBS. PBS/M-BmpP; oral administration with PBS for every 7 days and immunization with M-BmpB. WT/M-BmpP; oral administration with 2 × 108 CFU of WT-LAB for every 7 days and immunization with M-BmpP. sRANKL/M-BmpP; oral administration with 2 × 108 CFU of sRANKL-LAB every 7 days and immunization with M-BmpB. For significance tests, a one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test were used, and expressed as follows; *P < 0.05, **P < 0.01, ***P < 0.001