Fig. 3
Quantification of granulocyte subpopulations in the bone marrow, peritoneal cavity and oil granuloma after injection of pristane. Granulocyte cell subsets in the bone marrow, peritoneal cavity and oil granulomas were quantified by flow cytometry and characterized by morphological analysis. Grlow CD11bhi immature myeloid cells in bone marrow and peritoneal cavity (a and b, respectivelly) and Grhi CD11bhi mature granulocytes in bone marrow and peritoneal cavity (c and d, respectivelly) were quantified. In oil granulomas we observed an intense inflammatory infiltrate in both oil granulomas, however, in WT mice (e) this infiltrate was mainly composed by polymorphonuclear cells (insert), whereas in gal-3−/− animals (f) it was formed by mononuclear cells (insert). Data are reported as means ± standard error and are representative of 5 animals of each group. (*, P < 0.05). Black bars: WT animals; white bars: gal-3−/− animals