The effects of storage temperature on PBMC gene expression

BMC Immunology

Table 1 Number of genes significantly modulated by different storage conditions

Comparison	mRNA level change	Number of genes (FC > 2)	Number of genes (FC > 3)
Stored < −150 °C vs. Fresh	Increased	1453	450
Stored < −150 °C vs. Fresh	Decreased	1624	427
Stored ≤ −150 °C + Cycled vs. Fresh	Increased	1507	674
Stored ≤ −150 °C + Cycled vs. Fresh	Decreased	1721	420
Stored −80 °C vs. Fresh	Increased	1134	517
Stored −80 °C vs. Fresh	Decreased	2168	480
Stored ≤ −150 °C + Cycled vs. Stored ≤ −150 °C	Increased	0	0
Stored ≤ −150 °C + Cycled vs. Stored ≤ −150 °C	Decreased	0	0
Stored −80 °C vs Stored ≤ −150 °C	Increased	56	8
Stored −80 °C vs Stored ≤ −150 °C	Decreased	169	10

This table illustrates the number of genes with significantly (p < 0.05) higher or lower transcript levels following cryopreservation and thawing as compared to freshly isolated PBMCs or between storage conditions using an absolute Fold-Change cut-off of >2 (FC2) or >3 (FC3)

ISSN: 1471-2172