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Fig. 3 | BMC Immunology

Fig. 3

From: Double-stranded RNA analog and type I interferon regulate expression of Trem paired receptors in murine myeloid cells

Fig. 3

DAP12 binds Trem5 and Trem4 and facilitates Trem’s post-translational modification and cell-surface expression. a DAP12 is required for the post-translational modification of Trem5 and Trem4. Whole-cell lysates (WCL) prepared from HEK293FT cells transfected with the indicated combinations of HA-paired Trem proteins and/or FLAG-DAP12 were analyzed by immunoblotting with anti-FLAG or anti-HA Abs. b Association of DAP12 with paired Trem proteins. WCL prepared from HEK293FT cells transfected with the indicated combinations of HA-paired Trem proteins and FLAG-DAP12 were immunoprecipitated with anti-HA Ab and detected with anti-FLAG or anti-HA Abs. c DAP12 is required for cell surface expression of Trem5 and Trem4. Paired Trems were transfected into HEK293FT cells with or without DAP12. HA-paired Trem proteins on HEK293FT cells were analyzed using anti-HA mAb by FACS

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BMC Immunology

ISSN: 1471-2172

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