Fig. 2

Metallothionein expression in Tr1 cells is associated with increased Zn²⁺ release following exposure to H₂O₂ or DTDP. Intracellular Zn²⁺ release was measured in Tr1 cells from metallothionein knockout mice (MT^-/-) (n = 3) or congenic wildtype control mice (MT^+/+) (n = 3) exposed to different concentrations of H₂O₂ to measure oxidant-induced Zn²⁺ release. a The release of intracellular Zn²⁺ was measured by an increase in fluozin-3 fluorescence in wildtype Tr1 cells before and after exposure to H₂O₂ [5–40 μM] or (b) for metallothionein knockout (MT^-/-) Tr1 cells compared with wildtype controls exposed to H₂O₂ [40 μM] in one representative sample of each treatment is shown. c The increase in intracellular [Zn²⁺] after 7 min of H₂O₂ [1.25–40 μM] exposure was compared using linear regression. d The fold-increase in intracellular [Zn²⁺] after 100 μM DTDP exposure was compared using linear regression. Error bars indicate standard deviation