Fig. 3
From: Metallothionein regulates intracellular zinc signaling during CD4+ T cell activation
Metallothionein expression does not affect the total redox buffering capacity of Tr1 cells. CD4+ T cells from metallothionein knockout (MT-/-) (n = 4) or congenic wildtype control mice (MT+/+) (n = 4) were cultured for 0 (naïve) or 6 days in the presence of Il-27 (Tr1 effector). a Cell lysate was analyzed for the presence of free thiols by CPM assay and total protein concentration by BCA assay. b Tr1 cells were loaded with the oxidant sensitive probe CM-H2DCFDA. Fluorescence from the oxidized form of the probe was measured before and after H2O2 [50 μM] or PBS control exposure for 30 min to determine the intracellular redox buffering capacity. Bars indicate standard deviation. (*p < .05, **p < .01)