Fig. 5
From: Metallothionein regulates intracellular zinc signaling during CD4+ T cell activation
Metallothionein gene dose affects p38 MAPK activation in a Zn2+-dependent manner. Tr1 cells from metallothionein knockout (MT-/-) (n = 4) or wildtype control mice (MT+/+) (n = 4) were stimulated with (a) plate-bound anti-CD3 [5 μg/mL] or (b) 100 ng/mL PMA for 0–60 min. Cells were fixed, permeabilized and probed for the presence of phospho-p38. c During PMA induced activation, cells were treated with ZnSO4 [20 μM] with or without the zinc ionophore pyrithione, or with the zinc chelator (TPEN). Cells were fixed and permeabilized after 20 min and probed for and presence of phospho-p38. Error bars indicate standard deviation. (*p < .05)