Fig. 4

Serglycin proteoglycans influence neutrophil and eosinophil activity in T. spiralis infected mice (12 dpi). We assessed monocyte/macrophage (a) as well as neutrophil and eosinophil (b, c) recruitment in intestinal tissue in infected mice as compared with non-infected control mice, and measured the enzymatic activities of myeloperoxidase (MPO) (d) and neutrophil elastase (NE) (e) with substrate cleavage. We also quantified protein levels of NE and myeloblastin/proteinase 3 (PR3) (f) and eosinophil major basic protein (EMBP) (g) on western blots. In a data from two experiments are shown with control mice (N = 6) and with infected mice (N = 10). In b, c, d and e data from one representative experiment is shown with control mice (N = 3) and with infected mice (N = 3-5). In f and g a representative western blot is shown and a graph where the relative signal intensity (estimated with ImageJ) was calculated on N = 3-5 for each infected genotype and N = 3 for uninfected controls. Data is expressed as mean + SEM and significant differences are indicated in the figure. Not significant, ns P >0,05, **P <0.001, ***P <0.0001 versus infected WT mice, ##P <0.001, ###P <0.0001 versus infected mice