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Fig. 2 | BMC Immunology

Fig. 2

From: Existence of Th22 in children and evaluation of IL-22 + CD4 + T, Th17, and other T cell effector subsets from healthy children compared to adults

Fig. 2

Th22 cells exist in blood of young children and are memory CD4 + T cells. PBMCs from young children were stimulated with PMA and ionomycin for 4–6 h. Cell surface and intracellular staining was determined by FACS. a IL-22 + CD4 + T cells, IL-17 and IFN-γ production by IL-22 + CD4 + T cells from one representative donor were shown. CD4+ cells were gated from lymphocytes, and IL-22 producing CD4 + T cells were analyzed for IL-17 and IFN-γ production. b IL-22 production by CD45RO+ memory CD4 + T cells was shown from one representative donor. CD4 + T cells were gated from lymphocytes, and IL-22 and CD45RO expression was analyzed. c The statistical results of the percentages of IL-22 + cells by CD45RO+ or CD45RO- CD4 + T cells were shown (n = 7). The median is represented by horizontal line, the interquartile range by box and the minimum to maximum range by whiskers. *, p < 0.05

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BMC Immunology

ISSN: 1471-2172

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