Fig. 2
From: Avidity characterization of genetically engineered T-cells with novel and established approaches
Analysis of TCR-engineered T-cells. Human T-cells in a PBMC pool from healthy volunteers were activated and transduced with the pp65-TCR and TARP-TCR, respectively. The CD3+ T-cells were then evaluated for TCR expression by flow cytometry using multimer technologies and CD8 and CD4 staining. Expression of the transferred TCR was assessed seven days after lentiviral transduction (a), after multimer-guided magnetic bead sorting (b) and after an additional fourteen days of rapid expansion (c). The T-cells where analyzed after expansion in terms of percent TCR positive T-cells (d) and mean fluorescent intensity (e). SSC = side scatter