Fig. 4From: Intranasal immunization with recombinant Toxoplasma gondii actin depolymerizing factor confers protective efficacy against toxoplasmosis in miceCytokine concentrations of splenocytes from the immunized mice after stimulation with rTgADF. Splenocytes were collected from the mice two weeks after the last immunization and stimulated with 10 μg/mL of rTgADF. ELISA was used to examine the levels of IFN-γ (a), IL-2 (b), IL-4 (c), and IL-10 (d). Results are presented as means ± SD (pg/mL) for three independent experiments. *, P < 0.05 compared to the PBS group; #, P < 0.01 compared to the other immunized groupsBack to article page