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Fig. 6 | BMC Immunology

Fig. 6

From: Lipoproteins attenuate TLR2 and TLR4 activation by bacteria and bacterial ligands with differences in affinity and kinetics

Fig. 6

Effect of HSdelip or HSdelip supplemented with different apolipoproteins on the ability of bacteria to induce TLR2 activity. HEK-TLR2-TLR6 cells were incubated with S. typhimurium, E. coli, L. salivarius or B. breve at a ratio of 10:1 (black bars) or 25:1 (grey bars) (bact:cell) in the presence of HSdelip or HSdelip supplemented with the different apolipoproteins. ApoA enhanced, while ApoB and ApoC1 reduced, S. typhimurium (a) induced TLR2 activity, while ApoC2 and ApoC3 had no effect. ApoA and ApoC1-3, but not ApoB, were found to increase E.coli (b) or L.salivarius (c) induced TLR2 activity. No effect of ApoA and ApoC1-3 could be observed on B. breve (d) induced TLR2 activity, accept for ApoB which reduced TLR2 activity. Each value represents mean ± SD of triplicates. The experiment shown is representative of three separate experiments. . *:p < 0.05 **:P < 0.01 ***:P < 0.001 determined by comparison with HSdelip 10:1. $:p < 0.05 $$:P < 0.01 $$$:P < 0.001 determined by comparison with HSdelip 25:1

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