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Fig. 4 | BMC Immunology

Fig. 4

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Fig. 4

Cytokine profiles in TB-infected patient. Cytokine production by PPD- and ESAT-6-stimulated PBMC from a TB-infected donor was measured with kits from Millipore, Ozyme and Bio-Rad. a Specific cytokine Concentrations (background values subtracted). b Stimulation Index (ratio between stimulated and background concentration values). The value for IL-22 from non-stimulated PBMC given by the Millipore kit was inferior to the range of the standard curve and was therefore imputed by the lowest extrapolated value for this cytokine (3.1 pg/ml). The IL-17A concentration with ESAT-6 stimulation was a null value for all kits, so SI could not be calculated. The positive threshold for the SI was set at 3 (black line)

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