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Table 2 Intraclass correlation coefficients (ICCs) across HIV-stimulated samples

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Cytokine   FI Conca (pg/ml) Conc in range (pg/ml)
Culture duration Samples (n) Total meas. (n) ICC Samples (n) Total meas. (n) ICC Samples (n) Total meas. (n) ICC
IFNγ 11d 16 114 .69 16 107 .11 16 81 .51
Perforin 11d 16 91 .66 16 71 .17 16 61 .72
IL-5 11d 16 114 .36 16 106 .25 16 99 .23
IL-17 11d 16 114 .02 16 69 .02 16 28 .19
IFNγ 2d 17 135 .93 17 129 .88 17 122 .81
Perforin 2d 17 111 .16 17 97 NA 17 95 .88
IL-5 2d 17 135 .58 17 91 .55 17 86 .53
IL-17 2d 17 135 .43 17 37 .29 1 1 -
  1. ICCs were calculated across 9 replicate cultures. The combination ‘donor x stimulation’ was treated as one sample. d day, Conc concentration, meas measurement. ICCs > 0.8 are indicated in bold
  2. aincluding extrapolated values, NA: G matrix ‘not positive definite’