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Table 3 Working range and sensitivities

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Analyte Ozyme Millipore Bio-rad
theoretical experimental theoretical experimental theoretical experimental
LLOQ ULOQ LLOQ ULOQ LLOQ ULOQ LLOQ ULOQ LLOQ ULOQ LLOQ ULOQ
IL-17A 2.92 47900 2.90 12124a 9.60 30000 1.91 29561 1.78 29157 1.73 24822
IP-10 1.35 22100 1.10 5328a 1.60 5000 7.75a 4999 3.50 57400 3.12 52465
MCP-1 2.95 48400 2.60 12076a 1.60 5000 8.29a 5034 1.52 24920 1.64 24492
MIP-1α 0.52 8550 0.52 534b 1.60 5000 8.33a 5455 1.20 19707 1.21 3630a
MIP-1β 6.56 107500 24.58a 108640 1.60 5000 7.42a 4979 0.85 13960 1.03 10114
GM-CSF 4.29 70300 59.10b 70495 50.00 130000 10.00 117900 1.24 20314 16.27b 20993
IFNγ 0.20 3200 0.20 2323 8.00 25000 1.81 24966 2.13 34920 7.50a 34918
IL-10 0.34 5550 0.27 5424 2.40 7500 0.40 7502 1.59 26094 1.56 6551a
IL-13 2.89 47400 2.68 48745 6.40 20000 1.37 20019 2.40 39338 2.43 9065a
IL-22 13.47 220700 13.56 13752b 24.00 75000 4.66 75275 4.19 68313 4.22 17087a
IL-1β 0.71 11700 0.71 10791 4.80 15000 1.00 15029 1.90 31130 1.91 6893a
IL-2 0.40 6550 1.25a 7119 9.60 30000 1.92 30166 0.84 13690 3.09a 14356
IL-5 0.84 13800 3.28a 16674 5.60 17500 1.20 17052 2.34 38356 2.35 2392b
IL-6 0.92 15000 0.83 14549 3.20 10000 0.53 10233 1.54 25160 1.56 23428
TNFα 2.05 33600 1.87 2099b 3.20 10000 0.68 9999 6.54 107172 6.78 26507a
IL-12p40 3.34 54700 3.28 3092b 3.20 10000 15.46a 9965 4.72 77321 302.60c 77346
  1. Differences between theoretical and experimental limits of quantifications (LOQs) expressed in pg/ml are highlighted with a, b, and c for 1-dilution, 2-dilution and 3-dilution differences from theoretical LOQ, respectively