Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Table 3 Working range and sensitivities

Analyte	Ozyme				Millipore				Bio-rad
	theoretical		experimental		theoretical		experimental		theoretical		experimental
	LLOQ	ULOQ	LLOQ	ULOQ	LLOQ	ULOQ	LLOQ	ULOQ	LLOQ	ULOQ	LLOQ	ULOQ
IL-17A	2.92	47900	2.90	12124^a	9.60	30000	1.91	29561	1.78	29157	1.73	24822
IP-10	1.35	22100	1.10	5328^a	1.60	5000	7.75^a	4999	3.50	57400	3.12	52465
MCP-1	2.95	48400	2.60	12076^a	1.60	5000	8.29^a	5034	1.52	24920	1.64	24492
MIP-1α	0.52	8550	0.52	534^b	1.60	5000	8.33^a	5455	1.20	19707	1.21	3630^a
MIP-1β	6.56	107500	24.58^a	108640	1.60	5000	7.42^a	4979	0.85	13960	1.03	10114
GM-CSF	4.29	70300	59.10^b	70495	50.00	130000	10.00	117900	1.24	20314	16.27^b	20993
IFNγ	0.20	3200	0.20	2323	8.00	25000	1.81	24966	2.13	34920	7.50^a	34918
IL-10	0.34	5550	0.27	5424	2.40	7500	0.40	7502	1.59	26094	1.56	6551^a
IL-13	2.89	47400	2.68	48745	6.40	20000	1.37	20019	2.40	39338	2.43	9065^a
IL-22	13.47	220700	13.56	13752^b	24.00	75000	4.66	75275	4.19	68313	4.22	17087^a
IL-1β	0.71	11700	0.71	10791	4.80	15000	1.00	15029	1.90	31130	1.91	6893^a
IL-2	0.40	6550	1.25^a	7119	9.60	30000	1.92	30166	0.84	13690	3.09^a	14356
IL-5	0.84	13800	3.28^a	16674	5.60	17500	1.20	17052	2.34	38356	2.35	2392^b
IL-6	0.92	15000	0.83	14549	3.20	10000	0.53	10233	1.54	25160	1.56	23428
TNFα	2.05	33600	1.87	2099^b	3.20	10000	0.68	9999	6.54	107172	6.78	26507^a
IL-12p40	3.34	54700	3.28	3092^b	3.20	10000	15.46^a	9965	4.72	77321	302.60^c	77346

Differences between theoretical and experimental limits of quantifications (LOQs) expressed in pg/ml are highlighted with ^a, ^b, and ^c for 1-dilution, 2-dilution and 3-dilution differences from theoretical LOQ, respectively

ISSN: 1471-2172