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Table 4 Inter-assay variability of internal control

From: Optimization and evaluation of Luminex performance with supernatants of antigen-stimulated peripheral blood mononuclear cells

Plates IL-17F GM-CSF IFNγ IL-10 CCL20/MIP3α IL-13 IL-17A IL-22 IL-9 IL-2 IL-5 IL-27 TNFα
1 2091.2 7423.2 5300.1 81.4 9523.6 2248.3 74.0 45.4 225.6 89.8 191.9 808.9 3424.9
2 2059.3 6734.8 6833.8 82.0 9009.3 2537.1 90.9 65.0 256.9 107.0 224.2 1081.6 4126.7
3 1963.3 6811.0 5144.7 74.7 5706.4 2268.3 82.2 46.3 216.1 90.9 214.7 786.4 3571.6
4 2215.3 8326.4 5348.7 78.4 8241.1 2412.0 83.1 53.8 231.1 104.7 205.1 1308.3 3953.4
5 1998.1 6735.9 5506.4 61.1 8311.7 2064.6 72.1 48.5 195.9 89.2 172.9 1026.8 3385.1
6 2238.5 7407.3 6040.5 85.1 6113.7 2259.9 89.1 61.5 263.3 84.2 218.3 1093.6 4302.2
7 2473.7 8788.0 5809.2 71.3 8211.6 2560.0 88.3 47.0 278.8 98.5 243.6 1102.3 4128.4
8 2425.1 7440.4 5180.8 81.6 10147.7 2429.5 84.7 50.8 254.4 105.9 214.3 1190.2 4310.1
9 1945.9 6719.6 4545.8 68.4 8901.7 2384.8 82.4 53.3 198.0 95.8 221.2 1175.7 4241.0
10 2165.8 7817.6 7062.7 88.5 10767.6 2729.8 87.8 50.3 296.4 110.3 241.2 1070.5 5285.1
11 2185.4 6660.9 4907.7 68.6 8756.7 2387.7 70.6 39.6 180.1 91.9 170.5 914.5 3409.2
12 2151.4 7223.0 5796.1 65.0 8429.9 2304.6 71.1 42.6 224.8 86.8 195.3 1088.9 4515.4
13 2231.2 7964.7 5324.4 78.5 7028.9 2618.0 87.1 51.7 237.8 113.9 230.2 1249.2 4951.5
%CV 7.4 9.2 12.8 11.0 17.2 7.4 9.0 14.0 14.4 10.0 11.0 14.6 14.3
  1. Mean concentration values of duplicate Internal Controls (pg/ml) and %CVs of mean concentration calculated using the 13 inter-plates internal control mean concentration values for the selected 15-plex Millipore magnetic kit (see Material and Methods section for detailed analytes). IL-23 and IL-1β %CVs were not calculated due to concentration values < LLOQ and > ULOQ, respectively