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Table 1 Antibody Panels for Flow and Mass Cytometry Analyses

From: Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis

Antibody Clone Conjugate for detection Vendor
  FC MC FC MC FC MC
CD3 UCHT1 UCHT1 BUV737 170Er BD FL
CD4 RPA-T4 RPA-T4 PerCPCy5.5 145Nd BL FL
CD8a RPA-T8 RPA-T8 AF 700 146Nd BL FL
CD11b ICRF44 ICRF44 APC-Cy7 144Nd BL FL
CD11c Bu15 Bu15 AF 488 147Nd BL FL
CD14 HCD14 M5E2 BV 737 160Gd BD FL
CD15 W6D3 W6D3 BV 605 164Dy BD FL
CD16 n/a 3G8 n/a 148Nd n/a FL
CD19 HIB19 HIB19 AF 800 142Nd BL FL
CD25 2A3 2A3 PE 169Tm BD FL
CD27 L128 L128 BV 510 155Gd BD FL
CD33 n/a WM53 n/a 158Gd n/a FL
CD38 n/a HIT2 n/a 167Er n/a FL
CD44 n/a BJ18 n/a 166Er n/a FL
CD45 HI30 HI30 BUV395 154Sm BD FL
CD45RA n/a HI100 n/a 143Nd n/a FL
CD45RO n/a UCHL1 n/a 149Nd n/a FL
CD56 HCD56 HCD56 PE-Cy7 176Yb BL FL
CD62L n/a DREG-56 n/a 153Eu n/a FL
CD66b G10F5 80H3 AF 647 152Sm BD FL
CD66 n/a CD66a-B1.1 n/a 171Yb n/a FL
CD80 L307.4 n/a BV 510 n/a BD n/a
CD86 2331 IT2.2 BV 510 156Gd BD FL
CD107a n/a H4A3 n/a 151Eu n/a FL
CD127 A019D5 A019D5 BV 605 165Ho BL FL
CD152 n/a 14D3 n/a 161Dy n/a FL
CD183 n/a G025H7 n/a 156Gd n/a FL
CD185 n/a 51,505 n/a 171Yb n/a FL
CD194 n/a 205,410 n/a 158Gd n/a FL
CD196 n/a G034E3 n/a 141Pr n/a FL
CD197 n/a G043H7 n/a 159 Tb n/a FL
CD223 n/a 874,501 n/a 150Nd n/a FL
CD273 MIH18 24 F.10C12 BV 711 172Yb BD FL
CD274 29E.2A3 29E.2A3 BV 421 175Lu BL FL
CD279 EH12.2H7 EH12.2H7 BV 786 175Lu BL FL
CD357 621 In-house PE 159 Tb BL FL
TIGIT MBSA45 n/a AF 647 n/a eBio n/a
KI-67 n/a Ki-67 n/a 168Er n/a FL
HLA-DR L243 L243 PerCPCy5.5 174Yb BL FL
HLA-ABC G45-2.6 W6-32 PE-Cy7 141Pr BD FL
FOXP3 259D PCH101 AF 488 162Dy BL FL
  1. The antibodies used in both fluorescent and mass cytometry were commercially sourced
  2. Abbreviations: MC mass cytometry, FC flow cytometry, AF Alexa Fluor, BV Brilliant Violet, BUV Brilliant Ultra Violet, FL Fluidigm, BL BioLegend, BD BD Biosciences, eBio eBiosciences