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Fig. 1 | BMC Immunology

Fig. 1

From: An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity

Fig. 1

Determination of optimal concentrations of T-activated® pp65 and IE-1 for the stimulation of PBMC. PBMC were isolated from one CMV-seropositive healthy donor blood sample and stimulated for 19 h with increasing concentrations of T-activated® pp65 (31.6 fg/ml-31.6 μg/ml; black circles) or IE-1 (0.01-31.6 μg/ml; black squares). IFN-γ ELISpot results of four replicates are expressed as mean SFC/200,000 PBMC. Error bars represent standard deviations. The Y-axis scale was adjusted to pp65- (left) and IE-1- (right) specific values to optimize data resolution. Curve fitting was made using the four parameter logistic function of GraphPad Prism 5.04

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