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Fig. 8 | BMC Immunology

Fig. 8

From: An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity

Fig. 8

Assay validation in immunocompetent donors. PBMC isolated from whole blood of 45 healthy donors (Table 1) were assayed using the optimized IFN-γ ELISpot. A positivity cut-off of 10 SFC/200,000 PBMC (grey horizontal dashed line) was defined (see text and Additional File 2). Considering a test result as positive when the geometric mean for at least one of the IE-1 or pp65 stimulated approach is ≥ 10 SFC/200,000 PBMC and when the ratio of geometric means of stimulated to unstimulated conditions is ≥ 2.5, positive agreement (sensitivity) and negative agreement (specificity) of the optimized IFN-γ ELISpot test results with CMV serology within this collective of healthy donors was 97% and 85% respectively

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BMC Immunology

ISSN: 1471-2172

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