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Table 1 List of domain specific mutations in E protein of GI isolates in reference (Ref) to GIII strains

From: Insight into SNPs and epitopes of E protein of newly emerged genotype-I isolates of JEV from Midnapur, West Bengal, India

  

Genotype I (GI)

Genotype III (GIII)

E Protein

Mutations

AB051292

[Japan, Ishikawa, 1994]

JN703381

[WB, JEV28 2010]

JN703382

[WB, JEV21 2010]

KC526872

[WB, JEV45 2011]

3P54

SA14 virulent

M55506

SA14-14-2 Non-virulent

JN604986

Domain I

N--2H

.

+

+

+

N

N

N

N--2 T

+

.

.

.

N

N

N

Y183F

+

.

.

.

Y

Y

Y

R193K

+

.

.

.

R

R

K

K138Ea

(+)a

(+)a

(+)a

(+)a

Kb

E

K

V176Ia

(+)a

(+)a

(+)a

(+)a

Vb

I

V

A177Ta

(+)a

(+)a

(+)a

(+)a

T

T

A

Domain II

C-60Y

+

.

.

.

C

C

C

N103K

+

+

+

+

N

N

N

F107La

(+)a

(+)a

(+)a

(+)a

Fb

L

F

T129M

+

.

.

.

T

T

T

A222S

+

+

+

+

A

A

A

G244E

+

+

+

+

G

G

G

G261S

.

+

+

+

G

G

G

H264Qa

(+)a

(+)a

(+)a

(+)a

Q

Q

H

M279Ka

(+)a

(+)a

(+)a

(+)a

Mb

K

M

Domain III

V315A

+

+

+

+

V

V

V

S327T

+

+

+

+

S

S

S

A366S

+

.

.

.

A

A

A

V372L

+

.

+

+

V

V

V

M374I

+

.

+

+

M

M

M

G388K

+

.

+

+

G

G

G

W396R

+

.

+

+

W

W

W

Total mutations

21

13

17

17

4

6

Ref

Net mutations

15

7

11

11

-

-

 
  1. Amino acids marked with bsign in 3P54 have been identified as mismatches between PDB and UNIPROT sequence (P27395)
  2. All comparisons are made for first 406 residues of E protein (ecto domain). All three GI isolates of Midnapur/WB are taken with earlier Japan strain (Ishikawa). In GIII category, three sequences such as 3P54_A, virulent SA14 (ID M55506) and attenuated non-virulent SA14-14-2 (ID JN604986) are considered. + Sign indicates presence and dot indicates absence of mutations respectively. (+) indicates category of reported neurovirulent mutations [13]. Identical neurovirulent mutations of present isolates as SA14 are identified witha sign