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Table 1 List of domain specific mutations in E protein of GI isolates in reference (Ref) to GIII strains

From: Insight into SNPs and epitopes of E protein of newly emerged genotype-I isolates of JEV from Midnapur, West Bengal, India

   Genotype I (GI) Genotype III (GIII)
E Protein Mutations AB051292
[Japan, Ishikawa, 1994]
JN703381
[WB, JEV28 2010]
JN703382
[WB, JEV21 2010]
KC526872
[WB, JEV45 2011]
3P54 SA14 virulent
M55506
SA14-14-2 Non-virulent
JN604986
Domain I N--2H . + + + N N N
N--2 T + . . . N N N
Y183F + . . . Y Y Y
R193K + . . . R R K
K138Ea (+)a (+)a (+)a (+)a Kb E K
V176Ia (+)a (+)a (+)a (+)a Vb I V
A177Ta (+)a (+)a (+)a (+)a T T A
Domain II C-60Y + . . . C C C
N103K + + + + N N N
F107La (+)a (+)a (+)a (+)a Fb L F
T129M + . . . T T T
A222S + + + + A A A
G244E + + + + G G G
G261S . + + + G G G
H264Qa (+)a (+)a (+)a (+)a Q Q H
M279Ka (+)a (+)a (+)a (+)a Mb K M
Domain III V315A + + + + V V V
S327T + + + + S S S
A366S + . . . A A A
V372L + . + + V V V
M374I + . + + M M M
G388K + . + + G G G
W396R + . + + W W W
Total mutations 21 13 17 17 4 6 Ref
Net mutations 15 7 11 11 - -  
  1. Amino acids marked with bsign in 3P54 have been identified as mismatches between PDB and UNIPROT sequence (P27395)
  2. All comparisons are made for first 406 residues of E protein (ecto domain). All three GI isolates of Midnapur/WB are taken with earlier Japan strain (Ishikawa). In GIII category, three sequences such as 3P54_A, virulent SA14 (ID M55506) and attenuated non-virulent SA14-14-2 (ID JN604986) are considered. + Sign indicates presence and dot indicates absence of mutations respectively. (+) indicates category of reported neurovirulent mutations [13]. Identical neurovirulent mutations of present isolates as SA14 are identified witha sign