Fig. 6

T cells from anti-IL-7Rα-treated mice are sensitized for TCR-triggered co-inhibitory receptor induction. Female, prediabetic NOD mice (10–11 weeks) were treated with anti-IL-7Rα or rat IgG antibodies twice a week for 2 weeks. At the end of treatment, PLN were harvested and cells stimulated in vitro with anti-CD3 and anti-CD28 antibodies. a Representative dot plots (left) show percentages of LAG-3- and Foxp3-expressing cells within the CD4⁺ T cell gate 72 h after stimulation; summary (right) of LAG-3 expression levels (MFI) in the indicated cell populations stimulated with 0.1 μg/ml (white circles) or 10 μg/ml (black diamonds) anti-CD3. b Representative dot plots (left) and summary graph (right) show percentages of Tim-3⁺ CD44^high cells within the CD8⁺ T population, stimulated as indicated. Each symbol represents an individual mouse (n = 3–6). Data are representative for two independent experiments. * = p ≤ 0.05