Fig. 1
From: Recombinant Salmonella expressing SspH2-EscI fusion protein limits its colonization in mice
Construction of recombinant bacteria. a PCR products. M, Marker; 1, 5’-terminal of sspH2; 2, 3’-terminal of escI. b Recombinant plasmid pMD20 T-SspH2-EscI digested with Nco I and Sal I. M1-M2, Marker; 1, pMD20 T-SspH2-EscI. c Recombinant plasmid pYA3334-SspH2-EscI digested with Nco I and Sal I. M1-M2, Marker; 1, pYA3334-SspH2-EscI. d Growing curve of recombinant bacteria