Fig. 1

Representation of a scFv with the IMGT Labels for the two V-DOMAIN. The relative position 5′ and 3′ of the two V-DOMAIN in the scFv, in this figure, is arbitrary. At least 23 and 19 labels are necessary to describe a V-D-J-REGION and a V-J-REGION, respectively in IMGT/V-QUEST [59]. The conserved 1st-CYS 23, CONSERVED-TRP 41, 2nd-CYS 104 and J-TRP or J-PHE 118 based on the IMGT unique numbering are shown in both V-DOMAIN [1, 10, 14, 16]