Fig. 3

Frequencies of Tregs and correlations with virologic and immunologic indicators. PBMC from HIV uninfected controls (15), HIV recently (11) and chronically (eight) infected patients, were isolated and manipulated as described in methods section. These PBMC were stained with monoclonal antibodies and analyzed by polychromatic flow cytometry. Viral loads and frequency of antibodies reactive to HIV-1 proteins were determined using plasma and serum samples, respectively, as described on methods section. a-b Median and interquartile range for proportions and absolute counts of classic Tregs, CD4⁺CD25^HighFoxP3, in study groups. Each point represents an individual. Unpaired Mann-Whitney test p-values comparing medians from controls and HIV infected groups are indicated on each figure. c-g Correlations between the proportions of classic CD4⁺CD25^HighFoxP3⁺ Tregs with the viral loads, immune activation, absolute CD4 T cell counts and frequency of antibodies reactive to HIV-1 proteins at time of PBMC collections and at seroconversion visit. Each point corresponds to one individual. Spearman correlation r and p-values are indicated on each figure