Skip to main content
Fig. 10 | BMC Immunology

Fig. 10

From: Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy

Fig. 10

Complement-dependent-cytotoxicity of donor-specific antibodies from rats after syngeneic or allogeneic kidney transplantation treated with daily CyA for 6 or 28 days, or CyA on alternating days until d28. Donor splenocytes were incubated with recipient serum and rabbit complement. Dead cells were stained with propidium iodide (PI) and measured by flow cytometry. (a) shows representative histograms of PI-staining for negative control (cell medium, shown in red) and positive controls (Fix/perm, shown in blue; goat serum shown in green; rabbit serum shown in orange). (b) shows representative histograms of PI-staining for control (Lew d0), Txd6CyA, Txd28CyA, and Txd28CyAalt. (c) shows quantitative analysis as percent lysis (n = 3–4 per group). Significance (Mann-Whitney U Test) is shown as * p < 0.05 compared to control (Lewis rat serum pre-Tx d0), # p < 0.05 compared to “Txd28CyAalt”

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us