Fig. 2
From: Functional expression of CCL8 and its interaction with chemokine receptor CCR3
SDS-PAGE and circular dichroim analysis of CCL8 after digestion. a SDS-PAGE analysis of CCL8. Lane M represents protein standard markers. Lane 1 is His-tagged CCL8 fusion protein; lane 2, digested mixture of His-tagged CCL8 fusion protein; lane 3, purified CCL8 protein after the second Ni affinity chromatography; and lane 4 represents the mixture of undigested fusion protein, fusion part of CCL8 and His6-TEV enzymes captured on column, respectively. b Mass spectrometry analysis of the purified CCL8. c Circular dichroism analysis of recombinant CCL8