Fig. 4
Soluble factors derived from viral and bacterial-triggered NK cells increase the pro-inflammatory DC chemokine profile. NK cells (2.5 × 105 cells/well) were activated overnight in round-bottom 96-well plates with various viral and bacterial PAMPs (indicated on x-axis) in the presence of IL-2. As control, viral and bacterial PAMPs were stored overnight in the incubator without the presence of NK cells. iDC were matured in these cell-free NK cell-derived supernatants (gray bars) or control supernatants (white bars) supplemented with IL-4 (500 U/ml) and GM-CSF (500 U/ml). After 48 h, DC-derived chemokines were determined in the culture supernatant by CBA. a CCL5 production. b CXCL9 production. c CXCL10 production. Mean + SEM of n ≥ 3 independent experiments is shown. Mann-Whitney U test comparing absence and presence of NK cell-derived supernatant (white vs. gray bars). * P≤0.05, ** P≤0.01