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Fig. 1 | BMC Immunology

Fig. 1

From: The differences in immunoadjuvant mechanisms of TLR3 and TLR4 agonists on the level of antigen-presenting cells during immunization with recombinant adenovirus vector

Fig. 1

Immunoadjuvant effect of TLR3 and TLR4 agonists upon immunization with rAdTet-off H1 vaccine. Balb/c mice were immunized with 107 PFU rAdTet-off H1 alone or in combination with 10 μg LPS, 10 μg IMM or 5 μg Poly I:C. Forty days after immunization mice were euthanized, sorted populations of CD8+ (c) and CD4+ (d) T cells from spleens of immune mice were co-cultured with bone marrow DCs loaded with rAdTet-off H1 (35 PFU/cell) (a) or with recombinant protein H1 (2 μg/ml) (b), respectively. Reactivated IFNγ-producing T cells were detected using ELISPOT method. The number of IFNγ-positive spots is shown as M + SD (per 1 million spleen cells). Significant differences (p < 0.05) between the control group of mice immunized with rAdTet-off H1 (PBS) alone and groups of mice immunized with rAdTet-off H1 in combination with TLR agonists (LPS, IMM or Poly I:C) are indicated by asterisks

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BMC Immunology

ISSN: 1471-2172

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