Fig. 4

Impact of NLRP3-targeting siRNA on IL-1β expression. a, Inhibition rate of NLRP3, caspase-1 and IL-1β mRNA and IL-1β protein. The mRNA levels were measured by real-time PCR, and the proteins levels were measured via ELISA. b and c, Inhibitory rates of NLRP3, IL-1β and active caspase-1 proteins measured by western blotting analysis. The values represent the means±SDs of triplicate trials and are representative of three independent experiments. The western blotting results are representative of three independent experiments. One-way analysis of variance (ANOVA) was employed to examine differences between groups, and Dunnett’s post-comparison test was used to conduct multiple comparisons. NS, non-significant difference; *** P < 0.001