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Fig. 3 | BMC Immunology

Fig. 3

From: Blood handling and leukocyte isolation methods impact the global transcriptome of immune cells

Fig. 3

Effect of blood shipping and PBMC isolation methods on the global transcriptome of monocytes and T cells. CD8 T cells (CD3+CD8+) and Monocotyes (CD11b+CD66a) were isolated according to each condition and profiled by RNA-sequencing. a Principal components analysis (PCA) of the resulting transcriptomes (log2(FPKM+ 1) > 0.1) across all handling conditions. b Pairwise-scatter plots of the average log2(FPKM+ 1) were fit using linear regression and R2 values are shown

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