Fig. 5

Effect of mechanical isolation and preservation on the global transcriptome of monocytes and CD8 T cells. Blood was processed according to the sample simulation schematic for mechanical filtration and preservation method (CellCover). CD8⁺ T cells (CD3⁺CD8⁺) and monocotyes (CD11b⁺CD66a⁻) were isolated and profiled by RNA-sequencing. a Pairwise-scatter plots of the average log₂(FPKM+ 1) for each condition were fit using linear regression and R² values are shown. b Principal components analysis (PCA) of the resulting transcriptomes (log₂(FPKM+ 1) > 0.1) across all indicated conditions. Data were hierarchically clustered and the resulting cluster ID is shown on PCA plots