Fig. 4

Production of IL-6 in monocytes and their progenitors in wild type and Ceacam1 ^−/− mice. (a) Flow cytometry gating of monocytes (Mo), common monocyte progenitors (cMoP), monocyte-macrophage DC progenitors (MDP) and common DC precursors (CDP) in bone marrow cells (n = 4). (b) Percentage of Lin⁻ cells in bone marrow cells (n = 4). (Note: Lin = lineage markers: including CD3, CD19, B220, Ly6G, NK1.1, TER119) (c) Percentage of CD115⁺ cells in Lin⁻ bone marrow cells (n = 4). (d) Percentage of MDP, cMoP, Mo, and CDP in Lin⁻CD115⁺ bone marrow cells (n = 4). (e) Percentage of intracellular IL-6 staining in sorted MDP, cMoP, Mo, and CDP cells after treatment with Brefeldin A (BFA) plus 500 ng/mL LPS for 5 h in vitro (n = 4). (F) Flow analysis of intracellular IL-6 staining of sorted MDP, cMoP, Mo, and CDP cells after treatment with BFA plus 500 ng/mL LPS for 5 h in vitro (n = 4)